The NKI shRNA Library

shRNA library targeting 8000 human genes including: Cell cycle genes Oncogenes and tumorsuppressor genes Transcription factors Kinases and phosphatases Signal transduction Chromatin structure (43 genes) DNA repair and replication (71 genes) Adhesion Angiogenesis Apoptosis Genes implicated hereditary cancer Genes implicated drug response Disease genes etc... Generated 3 different shRNA constructs against each gene: 24.000 unique 19_mer sequences 24.000 pRETROSUPER shRNA constructs 48.000 59_mer oligos (~ 500 96 well plates oligos) ~ 80 96 well plates with bacterial glycerol stocks: each well 1 gene (3 shRNA constructs) each plate 96 genes (288 shRNA constructs) 4.000 shRNA constructs ( 8000 wells) DNA isolated from : single well / single gene (8000) per 96 well plate (~ 80 pools of 96 genes) subsets of genes of interest (gene families) total library